The high affinity of phahotoxins to filamentous actin (K, = 3.6 x 1O-8 M) has been used to assay small amounts of phallotoxins. The limit of detection was as low as 160 ng of phallotoxins. This is 50 times lower than the limit of all other procedures known so far. The assay was successfully applied
β¦ LIBER β¦
Phallotoxin and actin binding assay by fluorescence enhancement
β Scribed by Zhijian Huang; Rosaria P. Haugland; Weimin You; Richard P. Haugland
- Book ID
- 107719053
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 615 KB
- Volume
- 200
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A protein-binding assay for phallotoxins
β
A.J. SchΓ€fer; H. Faulstich
π
Article
π
1977
π
Elsevier Science
π
English
β 235 KB
The binding of fluorescent phallotoxins
β
Danuta Szczesna; Sherwin S. Lehrer
π
Article
π
1993
π
Springer Netherlands
π
English
β 568 KB
Evaluation of the binding of Acanthamoeb
β
Sara Lee; Min Li; Thomas D. Pollard
π
Article
π
1988
π
Elsevier Science
π
English
β 613 KB
We have used a fluorescence assay to measure the binding of Acanthamoeba profilin to monomeric Acanthamoeba and rabbit skeletal muscle actin labeled on cysteine-374 with pyrene iodoacetamide. The wavelengths of the pyrene excitation and emission maxima are constant at 346 and 386 nm, but the fluores
Microquantification of Cellular andin Vi
β
Vladimir L. Katanaev; Matthias P. Wymann
π
Article
π
1998
π
Elsevier Science
π
English
β 144 KB
Simultaneous assay for amatoxins and pha
β
F. Enjalbert; C. Gallion; F. Jehl; H. Monteil; H. Faulstich
π
Article
π
1992
π
Elsevier Science
π
English
β 707 KB
Silver nanoparticle-enhanced fluorescenc
β
Ji Li; Zhuying Wang; Ignacy Gryczynski; Wlodek Mandecki
π
Article
π
2010
π
Springer
π
English
β 442 KB