Evaluation of the binding of Acanthamoeb
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Sara Lee; Min Li; Thomas D. Pollard
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Article
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1988
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Elsevier Science
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English
β 613 KB
We have used a fluorescence assay to measure the binding of Acanthamoeba profilin to monomeric Acanthamoeba and rabbit skeletal muscle actin labeled on cysteine-374 with pyrene iodoacetamide. The wavelengths of the pyrene excitation and emission maxima are constant at 346 and 386 nm, but the fluores