𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A protein-binding assay for phallotoxins using muscle actin

✍ Scribed by A.J. Schäfer; H. Faulstich

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
235 KB
Volume
83
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

The high affinity of phahotoxins to filamentous actin (K, = 3.6 x 1O-8 M) has been used to assay small amounts of phallotoxins. The limit of detection was as low as 160 ng of phallotoxins. This is 50 times lower than the limit of all other procedures known so far. The assay was successfully applied to the analysis of phallotoxins in crude extracts of Amanita phalloides mushrooms. 720

📜 SIMILAR VOLUMES

A rapid assay procedure for thiamine-bin
A rapid assay procedure for thiamine-binding protein of Escherichia coli
✍ Takahiro Nishimune; Ryoji Hayashi 📂 Article 📅 1973 🏛 Elsevier Science 🌐 English ⚖ 275 KB

Although there are many methods available for detecting ligand binding to macromolecules (l-5), equilibrium dialysis is still the most commonly used. We report here a simple variation on equilibrium dialysis that greatly shortens the time needed for the assay of the thiaminebinding protein of E. col

Direct dye binding—A quantitative assay
Direct dye binding—A quantitative assay for solid-phase immobilized protein
✍ Martin Bonde; Hanne Pontoppidan; Duncan S. Pepper 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 375 KB

A direct dye-binding procedure was established for the quantification of protein after its immobilization on a solid phase, using IgG and BSA as model proteins. The assay, which in the range 0-5 mg protein/ml gel correlates well with indirect protein determination by A280 as well as determination of