Peroxidase activity and isoenzymes in the culture medium of NaCl adapted tomato suspension cells

✍ Scribed by María A. Sancho; Silvia Milrad Forchetti; Fernando Pliego; Victoriano Valpuesta; Miguel A. Quesada

• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 829 KB
• Volume: 44
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00048195

✦ Synopsis

The medium of tomato (Lycopersicon esculentum) cells adapted to grow in the presence of 15 g 1-1 NaC1 had a higher peroxidase activity than the medium of an unadapted tomato cell line. When the adapted cells were cultured in a medium without NaCI, the value found for peroxidase activity was intermediate. The increase in peroxidase activity was parallel to an increase of lignin-like compounds in the cell walls, as well as to an increased content or appearance of neutral and basic peroxidase isoenzymes. Apparently, the high values of peroxidase activity in the medium of the salt-adapted cells reflect the changed mechanical properties of the cell wall which, in turn, could be related to the salt adaptation process.

Abbreviations: L 0 -Control tomato cell line unable to grow in the presence of 15 g 1 -l ofNa C1; L15-tomato cell line adapted to 15 g 1-l of NaCI and growing in this salt concentration; L 15--0-tomato cell line adapted to 15 g 1-1 of NaCI and growing in the absence of this salt; 2,4-D-2,4-dichlorophenoxyacetic acid; N A A -naphthaleneacetic acid, PBS -phosphate buffer saline