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Peroxidase activity in calluses and cell suspension cultures of radishRaphanus sativusvar. Cherry Bell

✍ Scribed by Oscar A. Moreno V.; Rafael Vazquez-Duhalt

Publisher: Springer
Year: 1989
Tongue: English
Weight: 296 KB
Volume: 18
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00043401

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✦ Synopsis

Calluses of Raphanus sativus var. Cherry Bell were induced in a medium containing 2,4-dichlorophenoxyacetic acid and benzy|adenine. The biomass and peroxidase activities were determined, both in agar cultures and cell suspension cultures. Different growth-regulator concentrations induced different responses measured as peroxidase activity in callus. The suspended-cell cultures showed the importance of selecting the cell line, in order to obtain an optimal response in extracellular peroxidase activity. The commercial production of this enzyme by utilizing plant cell tissue cultures is discussed.

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