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Further studies on the relationship between the rates of nitrate uptake, growth and conductivity changes in the medium of plant cell suspension cultures

✍ Scribed by Klaus Hahlbrock

Publisher
Springer-Verlag
Year
1975
Tongue
English
Weight
456 KB
Volume
124
Category
Article
ISSN
0032-0935

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✦ Synopsis

The changes in packed cell volume and in nitrate content and conductivity of the medium during the growth cycle of cell suspension cultures from Petroselinum hortense Hoffm., Glycine max Merr., and Haplopappus gracilis A. Grey in a chemically defined medium were compared. In all three cases sigmoidal curves obtained for large decreases in the conductivity were paralleled by similar cm~ces for the rates of nitrate depletion from the medium until this nutrient was completely exhausted. Further decreases in the conductivity subsequent to nitrogen starvation proceeded at relatively slow rates and ceased when the cultures entered into the stationary phase of the growth cycle. Thus the previously reported method of deriving growth curves indirectly from conductivity measurements (Hahlbrock

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