The changes in packed cell volume and in nitrate content and conductivity of the medium during the growth cycle of cell suspension cultures from Petroselinum hortense Hoffm., Glycine max Merr., and Haplopappus gracilis A. Grey in a chemically defined medium were compared. In all three cases sigmoida
Determination of specific growth stages of plant cell suspension cultures by monitoring conductivity changes in the medium
✍ Scribed by Klaus Hahlbrock; Jürgen Ebel; Ann Oaks; John Auden; Manfred Liersch
- Publisher
- Springer-Verlag
- Year
- 1974
- Tongue
- English
- Weight
- 786 KB
- Volume
- 118
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Conductivity changes in the medium of cultured soybean (Glycine max L.) cells were shown to be strictly correlated with nitrate uptake and growth of the cultures. A continuous record of the conductivity was used as a simple and reliable method of determining specific growth stages and concomitant peaks in the activities of nitrate reductase and phenylalanine ammonia-lyase.
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