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Perfusion culture of hybridoma cells using a centrifuge to separate cells from culture mixture

✍ Scribed by Kimihiko Hamamoto; Kenji Ishimaru; Michiyuki Tokashiki

Book ID
113396100
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
461 KB
Volume
67
Category
Article
ISSN
0922-338X

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High density culture of hybridoma cells
High density culture of hybridoma cells using a perfusion culture vessel with an external centrifuge
✍ Michiyuki Tokashiki; Takami Arai; Kimihiko Hamamoto; Kenji Ishimaru πŸ“‚ Article πŸ“… 1990 πŸ› Springer 🌐 English βš– 368 KB

The influence of centrifugal force on the growth of cells was examined by exposing the cells of the mouse-human hybridoma X87 line to centrifugal force (100-500 G) for ten minutes twice a day and comparing the static culture with that of unexposed cells. In this experiment, both cell proliferation a

High density culture of mouse-human hybr
High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium
✍ Michiyuki Tokashiki; Takami Arai πŸ“‚ Article πŸ“… 1989 πŸ› Springer 🌐 English βš– 248 KB

Mouse-human hybridoma X87X ceils were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0 x 10 v cells/ml, when the