Five mouse-human hybridomas, H2, H3, V1, V2 and V6 cells secreting anti-virus human monoclonal immunoglobulin G (IgG) were cultured in serum-free media at high density in a settling perfusion culture vessel with an inner cell sedimentation zone. The H2, H3 and V6 cells reached a density of 107 cells
Acoustic Cell Filter for High Density Perfusion Culture of Hybridoma Cells
β Scribed by Trampler, Felix; Sonderhoff, Stefan A.; Pui, Phylis W. S.; Kilburn, Douglas G.; Piret, James M.
- Book ID
- 109902361
- Publisher
- Springer Science and Business Media LLC
- Year
- 1994
- Tongue
- English
- Weight
- 649 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0733-222X
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π SIMILAR VOLUMES
We have developed a high cell density and high product concentration culture system recycling high molecular weight components. The production of monoclonal antibodies in high concentration was performed by this culture system with mouse human hybridoma H2 and V6 cells in serum-free defined media.
The influence of centrifugal force on the growth of cells was examined by exposing the cells of the mouse-human hybridoma X87 line to centrifugal force (100-500 G) for ten minutes twice a day and comparing the static culture with that of unexposed cells. In this experiment, both cell proliferation a
## Abstract **BACKGROUND:** The production of Monoclonal antibodies (mAbs) is often performed in batch or fedβbatch operations where low cell densities and low volumetric productivities are achieved. The main bottleneck of both processes is the short operating time with productive cells at maximum