✦ LIBER ✦

Performance of nonrigid ion-exchange packings of small particle size in the separation of cytochrome c and derivatives by high-pressure liquid chromatography

✍ Scribed by Sj. van der Wal; J.F.K. Huber

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 617 KB
Volume: 105
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90448-0

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✦ Synopsis

Conditions for high-pressure liquid chromatography separations of cytochrome c and derivatives were investigated to provide an analytical and preparative tool for biochemical and medical research. Cation exchangers with a polystyrene-divinylbenzene or cellulose matrix were found to be not useful. Better results were obtained with hydroxylapatite and (meth)acrylic cation exchangers. The chromatographic selectivity with respect to cytochrome c derivatives is described at varying cation concentrations, pH of the eluent, and temperature. The column efficiency and its dependence on particle size for cytochrome c-like compounds is shown. A number of chromatograms is presented to prove the potential of this type of columns.

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