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Determination of CoASH by high-performance liquid chromatography and its application in the assay of long-chain acyl-CoA derivatives

โœ Scribed by Ole C. Ingebretsen; Per T. Normann; Torgeir Flatmark

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
611 KB
Volume
96
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A high-performance liquid chromatographic procedure is described for the determination of picomole amounts of CoASH, using a microparticulate, strong anion-exchange resin. The method was applied in a systematic study to optimize the conditions for alkaline hydrolysis of palmitoyl-CoA. The procedure, which ensures 100% recovery of CoASH by hydrolysis of palmitoyl-CoA, was found to be convenient also for the assay of the endogenous content of long-chain acyl-CoA derivatives in biological material.

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## Abstract A simple, specific and sensitive HPLC method with UV detection was developed and validated for the determination of tectoridin in rat plasma for the first time. Chromatographic separation was performed on a Welchrom^TM^ C~18~ column (150 ร— 4.6ย mm, i.d., 5ย ยตm) at a flow rate of 1.0ย mLย mi