Determination of tectoridin in rat plasma by high-performance liquid chromatography and its application to pharmacokinetic studies

✍ Scribed by Taiming Liu; Zhuo Chen; Yuyi Guo; Xuehua Jiang; Guo Ma

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 148 KB
• Volume: 23
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1180

✦ Synopsis

Abstract

A simple, specific and sensitive HPLC method with UV detection was developed and validated for the determination of tectoridin in rat plasma for the first time. Chromatographic separation was performed on a Welchrom^TM^ C~18~ column (150 × 4.6 mm, i.d., 5 µm) at a flow rate of 1.0 mL min^−1^, using a mixture of methanol–2% HAc aqueous solution (31:69, v/v) as the mobile phase with UV detection at 266 nm. The calibration curves for tectoridin were linear over the concentration range of 1.10–274.40 µg mL^−1^ in rat plasma. The intra‐ and inter‐day accuracies (RE) were within −3.23% and 4.11%. The intra‐ and inter‐day precisions (RSD) were not more than 2.74 and 4.72%, respectively. The present method was successfully applied to the pharmacokinetic studies of tectoridin in rats after intravenous administration of three different doses. Copyright © 2009 John Wiley & Sons, Ltd.