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PEG + Potassium Phosphate + Urea Aqueous Two-Phase Systems: Phase Equilibrium and Protein Partitioning

✍ Scribed by Meller da Silva, Luiza H.; Meirelles, Antonio J. A.


Book ID
126837192
Publisher
American Chemical Society
Year
2001
Tongue
English
Weight
55 KB
Volume
46
Category
Article
ISSN
0021-9568

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An aqueous two-phase system of polyethylene glycol (PEG) and potassium phosphate was developed for extraction of a cutinase from cell debris of a recombinant Escherichia coli strain. Basic studies to identify the primary factors which affect cutinase partition, namely the influence of polymer molecu