The instrument used for analytical isotachophoresis in these experiments was the LKB 2127 Tachophor. The addition of a detergent greatly increased the sharpness of the zone boundaries. The resolution of the UV detector was found to be approx. 0.1 mm zone length, corresponding to a volume of 16 nl. W
Peak capacity and resolution in capillary columns
β Scribed by Said, A. S.
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 251 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0935-6304
No coin nor oath required. For personal study only.
β¦ Synopsis
This series is intended to present the basic theory of chromatography in simple and clear mathematics which can be readily followed by the average chemist. The basic ideas are not necessarily novel but the approach and applications should be original and of interest to a large number of workers in chromatography rather than being limited to a small number of highly qualified chromatographers of great mathematical ability.
π SIMILAR VOLUMES
## Abstract A rapid and specific procedure is described for the resolution of nine steroids present in conjugated estrogen preparations. Resolution is achieved on a short glass capillary column coated with Silar 10C after dual derivatization to oximeβtrimethylsilyl ethers.
The elution profiles observed with overloaded capillary columns are well described by a four-parameter theoretical model derived from the basic mass balance equations which relies on an expansion of the partition isotherm to the second term and takes the sorption effect into account. The reliability
## Abstract Many uncoated precolumns, and to a lesser extent separation columns, strongly retain some of the solvent. Retarded release of such solvent elevates the baseline after the solvent peak and causes ugly βhumpsβ of eluted solvent as the temperature is increased. The problem is probably a re