## Abstract Glass capillary columns coated with OVโ225 and Silar 10C were used to analyze estrogens (as their trimethylsilyl derivatives) present in pregnant mares' urine. Such columns provide better resolution in a shorter time than the corresponding packed columns, as exemplified by the separatio
Resolution of equine estrogens using glass capillary columns
โ Scribed by Pillai, G. K. ;McErlane, K. M.
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 320 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0935-6304
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โฆ Synopsis
Abstract
A rapid and specific procedure is described for the resolution of nine steroids present in conjugated estrogen preparations. Resolution is achieved on a short glass capillary column coated with Silar 10C after dual derivatization to oximeโtrimethylsilyl ethers.
๐ SIMILAR VOLUMES
CP-Sil-5-CB (20 m, 0.25 mm i.d.) 200 "C -O.S"/min ~ 215 "C ' ) CP-Sil-19-CB (25 m, 0.25 mm i.d.) 225 "C -0.2"/min -229 "C ~ 8"/min ~ 261 "C -4"/min -275 "C. dl CP-Sil-5-CB (10 m, 0.25 mm i.d.) CP-Sil-19-CB (10 m, 0.32 mm i.d.) 190 "C ~ l"/min ~ 250 "C. e, CP-Sil-5-CB (10 m. 0.25 mm i.d.) CP-Sil-19-C
## Abstract The performance of graphitized glass capillary columns obtained by precoating the inner glass walls with graphitized carbon black has been evaluated. It has been shown that according to the amount of stationary phase coated such columns can operate in gasโliquidโsolid chromatography and
It is well known that removal of metal ionsfrom the glass surface greatly improves both the thermal stability and the adsorption characteristics of glass capillary columns.Usually metal ions are removed from a glass surface by acid leaching; in particular, the static leaching procedure with hydrochl
## Abstract The proposed combination of a rather simple procedure for sample preparation with capillary gasโliquid chromatography using a barium carbonate/polyethyleneglycol 20,000 column and a Grobโtype onโcolumn injector permits measurement of bile acids in serum with high separation efficiency,