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Analytical isotachophoresis : Resolution, detection limits and separation capacity in capillary columns

✍ Scribed by Lennart Arlinger

Publisher: Elsevier Science
Year: 1974
Tongue: English
Weight: 763 KB
Volume: 91
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(01)97960-9

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✦ Synopsis

The instrument used for analytical isotachophoresis in these experiments was the LKB 2127 Tachophor. The addition of a detergent greatly increased the sharpness of the zone boundaries. The resolution of the UV detector was found to be approx. 0.1 mm zone length, corresponding to a volume of 16 nl. With leading ion concentrations of 10 mM and 0.5 mM, the detection limits were about 25 and l-2 pmoles ATP, and the correct UV absorbance levels were reached at about 125 and 50 pmoles ATP, respectively.

Current and temperature had little influence on the separation capacity. The separation capacity as a function of capillary length, sample volume and counter-flow of leading electrolyte was also studied.

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