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Partial purification and properties of long-chain acyl-CoA hydrolase from rat brain cytosol

✍ Scribed by Augustine Y. Lin; Grace Y. Sun; Ron macQuarrie


Publisher
Springer
Year
1984
Tongue
English
Weight
937 KB
Volume
9
Category
Article
ISSN
0364-3190

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A method for the extraction of acyl-CoA esters from tissue, and their subsequent analysis by HPLC is described. The lipids are removed by a two-phase extraction in a chloroform/methanol/water system. The long-chain acyl-CoA esters are extracted using methanol and a high salt concentration (2 M ammon