Partial fractionation and characterization of nuclear protein kinases in HeLa S3 cells
โ Scribed by Judith A. Thomson; Manuel J. Mon; Janet L. Stein; Kathryn A. Duval; Lewis J. Kleinsmith; Gary S. Stein
- Book ID
- 113210086
- Publisher
- Elsevier Science
- Year
- 1979
- Weight
- 962 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0045-6039
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๐ SIMILAR VOLUMES
The rate of DNA synthesis in HeLa S3 cells, as measured by incorporation of C14-labeled thymidine, is strongly dependent on protein synthesis at all times during the S phase. The relation between the rate of DNA synthesis and the rate of protein synthesis is linear when measured two or three hours a
In this study we investigated the effect of heat on the proteins of the particulate fraction (PFj of HeLa S3 cells using electron spin resonance (ESRj and thermal gel analysis (TCA). ESR detects overall conformational changes in proteins, while TG.4 detects denaturation (aggregation due to formation