Fractionation and partial purification of rat liver nuclear protein kinases
โ Scribed by Said A. Goueli; Khalil Ahmed
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 924 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0020-711X
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Damage to DNA can result in strand breaks with 58-hydroxyl and 38-phosphate termini. Before DNA polymerases and ligases can rejoin the broken strands, such termini have to be restored to 58-phosphate and 38-hydroxyl groups. Polydeoxynucleotide kinase is an enzyme that may fulfil this function. We ha
## Abstract Nuclear proteins of rat liver and rat ascites hepatoma were fractionated by extraction in solutions of different salt concentration and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The difference between the electrophorograms of the different fractions of nucle