Solubilization and characterization of microsomal protein kinases from rat liver
โ Scribed by Eng L. Tan; Clinton Leinweber; Katherine F. Lewis; Chung S. Yang
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 800 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0020-711X
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๐ SIMILAR VOLUMES
A lipid transfer protein was isolated from bovine liver. Following the release of soluble proteins from liver microsomes, the transfer protein was purified 75-fold to near homogeneity by a combination of DEAE-celiulose ion exchange, Sephadex G-200 gel permeation, and hydroxylapatite chromatography.
A simple, efficient, freeze-thaw procedure for the solubilization of liver 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase has been developed. Microsomes of chicken or rat liver were prepared by homogenization in buffer containing 100 mM sucrose, 50 mM KCI, 40 mM KH,PO,, 30 mM EDTA. and 2