✦ LIBER ✦

The dependence of DNA synthesis on protein synthesis in HeLa S3 cells

✍ Scribed by Barbara G. Weiss

Publisher: John Wiley and Sons
Year: 1969
Tongue: English
Weight: 471 KB
Volume: 73
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040730112

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✦ Synopsis

The rate of DNA synthesis in HeLa S3 cells, as measured by incorporation of C14-labeled thymidine, is strongly dependent on protein synthesis at all times during the S phase. The relation between the rate of DNA synthesis and the rate of protein synthesis is linear when measured two or three hours after reducing the rate of protein synthesis with either puromycin or cycloheximide. The effect is manifested rapidly, is found in both random and synchronized cultures, and is independent of the method of synchronization.

DNA synthesis in mammalian cells occurs during a discrete portion of the cell cycle and is thought to involve the sequential activation and duplication of a large number of replicating units (Stubblefield and Mueller, '62; Painter et al., '66; Okada, '68). Studies with bacteria have shown that protein synthesis is essential

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