✦ LIBER ✦

BIFUNCTIONAL EFFECTS OF HEPARIN-BINDING PROTEIN HBp17 ON DNA SYNTHESIS IN CELLS

✍ Scribed by Jun-Hui Chen; Xin Chang Wang; J.Denry Sato

Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 85 KB
Volume: 25
Category: Article
ISSN: 1065-6995
DOI: 10.1006/cbir.2000.0688

No coin nor oath required. For personal study only.

✦ Synopsis

A 17kD heparin‐binding protein (HBp17) has a biphasic dose‐dependent effect on DNA synthesis in 3T3 cells. Maximal stimulation of DNA synthesis occurs at 8ng/ml HBp17, but a half‐maximal inhibition occurs at ∼500ng/ml. This inhibition can easily be reversed by addition of 400pg/ml aFGF or 100pg/ml bFGF, whereas EGF had no effect. This biphasic action of HBp17 was also seen in human umbilical vein endothelial cells (HUVEC), whereas it was not found in the malignant cell line, A431‐AJC. The functional relationship between HBp17 and FGF is discussed.

📜 SIMILAR VOLUMES

The dependence of DNA synthesis on prote

The dependence of DNA synthesis on protein synthesis in HeLa S3 cells

✍ Barbara G. Weiss 📂 Article 📅 1969 🏛 John Wiley and Sons 🌐 English ⚖ 471 KB 👁 1 views

The rate of DNA synthesis in HeLa S3 cells, as measured by incorporation of C14-labeled thymidine, is strongly dependent on protein synthesis at all times during the S phase. The relation between the rate of DNA synthesis and the rate of protein synthesis is linear when measured two or three hours a

Effects of extracellular calcium concent

Effects of extracellular calcium concentration on protein synthesis in Aedes albopictus cells

✍ Marcia Tie Kawamura; Maria da Gloria da Costa Carvalho 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 128 KB 👁 2 views

The influence of extracellular calcium concentration on mosquito cells was investigated in Aedes albopictus cells cultured in a medium with different amounts of calcium. Protein synthesis in cells incubated in low calcium culture medium was inhibited when compared to control cells. This inhibition w

Effects of extracellular calcium concent

Effects of extracellular calcium concentration on protein synthesis in Aedes albopictus cells

✍ Marcia Tie Kawamura; Maria da Gloria da Costa Carvalho 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 118 KB 👁 2 views

Effect of heparin on bilirubin clearance

Effect of heparin on bilirubin clearance in rats: Pharmacokinetic consequences of extensive hepatic extraction of plasma protein binding inhibitors

✍ Ulf W. Wiegand; David M. Soda; Gerhard Levy 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 416 KB 👁 1 views

Effects of growth rate on cell extract p

Effects of growth rate on cell extract performance in cell-free protein synthesis

✍ James Zawada; James Swartz 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 146 KB 👁 2 views

## Abstract Cell‐free protein synthesis is a useful research tool and now stands poised to compete with in vivo expression for commercial production of proteins. However, both the extract preparation and protein synthesis procedures must be scaled up. A key challenge is producing the required amoun

Effect of vitamin C on prostate cancer c

Effect of vitamin C on prostate cancer cells in vitro: Effect on cell number, viability, and DNA synthesis

✍ Maramag, Carlos; Menon, Mani; Balaji, K.C.; Reddy, Premveer G.; Laxmanan, Seetha 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 166 KB 👁 2 views

## BACKGROUND. Many studies describe the protective role of vitamin C (ascorbic acid) against cancer development and in treatment of established cancer. The present study investigated whether ascorbic acid demonstrates a therapeutic benefit for prostate cancer. METHODS. Androgen-independent (DU145