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Partial filling multiple injection affinity capillary electrophoresis (PFMIACE) to estimate binding constants of receptors to ligands

โœ Scribed by Jose Zavaleta; Dinora B. Chinchilla; Alejandra Ramirez; Amaris Pao; Karla Martinez; Sanjay Nilapwar; John E. Ladbury; Sanku Mallik; Frank A. Gomez


Book ID
116901844
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
761 KB
Volume
71
Category
Article
ISSN
0039-9140

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Flow-through partial-filling affinity ca
โœ John Kaddis; Erica Mito; Joseph Heintz; Alfredo Plazas; Frank A. Gomez ๐Ÿ“‚ Article ๐Ÿ“… 2003 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 109 KB ๐Ÿ‘ 1 views

This work evaluates the use of a competitive binding assay using flow-through partial-filling affinity capillary electrophoresis (FTPFACE) to estimate binding constants of neutral ligands to a receptor. We demonstrate this technique using, as a model system, carbonic anhydrase B (CAB, EC 4.2.1.1) an