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Multiple-injection affinity capillary electrophoresis to estimate binding constants of receptors to ligands

โœ Scribed by Dinora Chinchilla; Jose Zavaleta; Karla Martinez; Frank A. Gomez


Publisher
Springer
Year
2005
Tongue
English
Weight
180 KB
Volume
383
Category
Article
ISSN
1618-2650

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Flow-through partial-filling affinity ca
โœ John Kaddis; Erica Mito; Joseph Heintz; Alfredo Plazas; Frank A. Gomez ๐Ÿ“‚ Article ๐Ÿ“… 2003 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 109 KB ๐Ÿ‘ 1 views

This work evaluates the use of a competitive binding assay using flow-through partial-filling affinity capillary electrophoresis (FTPFACE) to estimate binding constants of neutral ligands to a receptor. We demonstrate this technique using, as a model system, carbonic anhydrase B (CAB, EC 4.2.1.1) an