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Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis

โœ Scribed by Li-Wei Zhang; Li Ding; Xin-Xiang Zhang


Publisher
Springer
Year
2007
Tongue
English
Weight
234 KB
Volume
387
Category
Article
ISSN
1618-2650

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Flow-through partial-filling affinity ca
โœ John Kaddis; Erica Mito; Joseph Heintz; Alfredo Plazas; Frank A. Gomez ๐Ÿ“‚ Article ๐Ÿ“… 2003 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 109 KB ๐Ÿ‘ 1 views

This work evaluates the use of a competitive binding assay using flow-through partial-filling affinity capillary electrophoresis (FTPFACE) to estimate binding constants of neutral ligands to a receptor. We demonstrate this technique using, as a model system, carbonic anhydrase B (CAB, EC 4.2.1.1) an