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Partial characterization of β-glucosidase activity produced byKluyveromyces marxianusIMB3 during growth on cellobiose-containing media at 45°C

✍ Scribed by N. Barron; R. Marchant; L. McHale; A. P. McHale

Publisher
Springer Netherlands
Year
1995
Tongue
English
Weight
280 KB
Volume
17
Category
Article
ISSN
0141-5492

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✦ Synopsis

We report-the partial characterization of a 13-glucosidase produced during growth of the thermotolerant yeast, K. marxianus IMB3 on lactose-containing media at 45°C. The enzyme had Km values of 1.1mM and 14.SmM fbr the substrates p-nitrophenyl-13-D-glucoside and ceilobiose, respectively. The enzyme had a pH optimum of 5.5 and was optimally active at 50°C. It was stable up to 125 hours at 25°(3 and 35 °, with half-lives of 45 hours and 2 hours at 45°C and 50°C, respectively. The enzyme was inhibited to varying degrees in the presence of metal ions and was completely inactivated by Hg 2+. Ethanol concentrations [1-10% (v/v)] had little effect on activity. Glucose (20mM) caused inhibition when p-nitrophenyl-13-D-glucoside was used as substrate, whereas lactose at similar concentrations had no effect.

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The effects of Mn2+on ethanol production
The effects of Mn2+on ethanol production byKluyveromyces marxianusIMB3 during growth on lactose-containing media at 45°C
✍ D. Brady; R. Marchant; L. McHale; A. P. McHale 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 228 KB

It has previously been demonstrated that the thermotolerant yeast strain, K. marxianus IMB3 is capable of growth and ethanol production on lactose containing media at 45°C. Although the organism is capable of producing a ~-galactosidase, that enzyme has been shown to be extremely thermolabile at 45