It has previously been demonstrated that the thermotolerant yeast strain, K. marxianus IMB3 is capable of growth and ethanol production on lactose containing media at 45°C. Although the organism is capable of producing a ~-galactosidase, that enzyme has been shown to be extremely thermolabile at 45
✦ LIBER ✦
Increased ethanol production during growth of electric-field stimulatedKluyveromyces marxianusIMB3 during growth on lactose-containing media at 45°C
✍ Scribed by J. Simpson; D. Brady; A. Rollan; N. Barron; L. McHale; A. P. McHale
- Publisher
- Springer Netherlands
- Year
- 1995
- Tongue
- English
- Weight
- 302 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0141-5492
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We report-the partial characterization of a 13-glucosidase produced during growth of the thermotolerant yeast, K. marxianus IMB3 on lactose-containing media at 45°C. The enzyme had Km values of 1.1mM and 14.SmM fbr the substrates p-nitrophenyl-13-D-glucoside and ceilobiose, respectively. The enzyme