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Paclitaxel-induced apoptosis in HeLa cells is serum dependent

✍ Scribed by Maldonado, Vilma ;De Anda, Jose ;Meléndez-Zajgla, Jorge

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
181 KB
Volume
11
Category
Article
ISSN
0887-2082

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✦ Synopsis

Exposure of HeLa cells to different concentrations of the antineoplastic drug paclitaxel resulted in a loss of cell viability that was dependent on the concentration and time of exposure to the drug. This phenomenon was associated with the appearance of nuclear morphology typical of apoptosis and DNA breakage into a "ladder" pattern of discrete fragments of nucleosomal size. The induction of cell death was dependent on the serum concentration of the culture media, repressed by pretreatment with a cAMP-dependent protein kinase (PKA) inhibitor, and enhanced by increasing the cell proliferation with previous exposure to a cAMP-analog and a protein kinase-C (PKC) inducer. The proliferative index modifies the effect of taxol on HeLa cells, probably by means of a more rapid accumulation of cells in the G2/M cycle blockage point, although a direct participation of PKA and PKC should not be excluded.

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