Fluchloralin is cytotoxic and genotoxic and induces apoptosis in mammalian cells

✍ Scribed by S. Sinha; N. Panneerselvam; G. Shanmugam

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 330 KB
• Volume: 31
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/(sici)1098-2280(1998)31:3<257::aid-em7>3.0.co;2-h

✦ Synopsis

The genotoxic and cytotoxic effects of a widely used To understand the mechanism of cell death caused herbicide, fluchloralin, were assessed using cultured by fluchloralin, its effect on DNA synthesis and its mammalian cells. Treatment of cells for 8-12 hr with ability to induce apoptosis were investigated. Even fluchloralin resulted in a significant increase in the short (6 hr) treatment of cells with fluchloralin resulted frequency of metaphase cells with chromosomal dam-in a 30-50% inhibition of DNA synthesis. Agarose age. At higher concentrations, the herbicide also in-gel electrophoresis of DNA from herbicide-treated duced an increase in the frequency of sister chroma-cells and cytochemical staining indicate the induction tid exchange. A 50% loss in viability was observed of apoptosis by fluchloralin. Environ. Mol. Mutagen. when cells were exposed to the herbicide for 72 hr.