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Paclitaxel-induced apoptosis in MCF-7 breast-cancer cells

✍ Scribed by Dwight E. Saunders; W. Dwayne Lawrence; Carl Christensen; Nanci L. Wappler; Hangming Ruan; Gunter Deppe

Publisher: John Wiley and Sons
Year: 1997
Tongue: French
Weight: 180 KB
Volume: 70
Category: Article
ISSN: 0020-7136
DOI: 10.1002/(sici)1097-0215(19970117)70:2<214::aid-ijc13>3.0.co;2-i

No coin nor oath required. For personal study only.

✦ Synopsis

A study of MCF-7 human breast cancer cells was undertaken to ascertain the degree of apoptosis induction by paclitaxel and if the induction of apoptosis could be enhanced by caffeine. Paclitaxel (0-20 ng/ml) caused concentration-dependent increases in morphologically identifiable apoptotic cells (up to 43% of cell population) and cells with DNA strand breaks (up to 38%), a commonly cited marker of apoptosis. Maximal DNA strand breakage occurred after 16 hr of exposure to paclitaxel and maximal apoptotic-appearing cells occurred after 24 hr. The remaining non-apoptotic paclitaxel-exposed cells were growth arrested in G2. A 4-hr exposure to caffeine concentration-dependently (0-20 mM) increased apoptosis to 88% of the cell population. Our results show induction of apoptosis in breast cancer cells by paclitaxel, and enhancement of this process by caffeine.

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