Oxygen requirements for growth and citric acid production of Yarrowia lipolytica

✍ Scribed by Svetlana V Kamzolova; Nadezda V Shishkanova; Igor G Morgunov; Tatyana V Finogenova

• Book ID: 104443542
• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 185 KB
• Volume: 3
• Category: Article
• ISSN: 1567-1356
• DOI: 10.1016/s1567-1356(02)00188-5

✦ Synopsis

During continuous cultivation of Yarrowia lipolytica N 1, oxygen requirements for growth and citric acid synthesis were found to depend on the iron concentration in the medium. A coupled effect of oxygen and iron concentrations on the functioning of the mitochondrial electron transport chain in Y. lipolytica N 1 was established. Based on the results obtained in continuous culture, conditions for citric acid production in a batch culture of Y. lipolytica N 1 were proposed. At relatively low pO(2) value and a high iron concentration, citric acid accumulation was as high as 120 g l(-1); the specific rate of citric acid synthesis reached 120 mg citric acid (g cells h)(-1). The mass yield coefficient was 0.87 and the energy yield coefficient was 0.31.