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Oxalate accumulation from citrate byAspergillus niger

✍ Scribed by Hans-Martin Müller

Publisher
Springer
Year
1975
Tongue
English
Weight
555 KB
Volume
103
Category
Article
ISSN
0302-8933

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✦ Synopsis

Carbon-i4 was incorporated from citrate-/,5-14C, glyoxylate-14C(U), or glyoxylate-l-a4C into oxalate by cultures of Aspergillus niger pregrown on a medium with glucose as the sole source of carbon. Glyoxylate-14C(U) was superior to glyoxylate-l-14C and citrate-l,5-~4C as a source of incorporation. By addition of a great amount of citrate the accumulation of oxalate was accelerated and its maximum yield increased. In a cell-free extract from mycelium forming oxalate from citrate the enzyme oxaloacetate hydrolase (EC3.7.1.1) was identified. Its in vitro activity per flask exceeded the rate of in vivo accumulation of oxalate. Glyoxylate oxidizing enzymes (glycolate oxidase, EC 1.1.3.1; glyoxylate oxidase, EC 1.2.3.5; NAD(P)-dependent glyoxylate dehydrogenase; glyoxylate dehydrogenase, CoA-oxalylating, EC 1.2.1.17) could not be detected in cellfree extracts. It is concluded that in cultures accumulating oxalate from citrate after pregrowth on glucose, oxalate arises by hydrolytic cleavage of oxaloacetate but not by oxidation of glyoxylate.

📜 SIMILAR VOLUMES

Oxalate accumulation from citrate byAspe
Oxalate accumulation from citrate byAspergillus niger
✍ H. -M. Müller; S. Frosch 📂 Article 📅 1975 🏛 Springer 🌐 English ⚖ 463 KB

Carbon-14 was incorporated into oxalate and CO2 from either citrate-l,5-14C, succinate-l,4-14C, or fumarate-l,4-14C by cultures of Aspergillus niger pregrown on a medium which contained glucose as the sole carbon source and which did not allow citrate accumulation. In cell-free extracts of mycelium