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Overexpression of the proto-oncogene/translation factor 4E in breast-carcinoma cell lines

✍ Scribed by Bruce Anthony; Peggy Carter; Arrigo De Benedetti

Publisher: John Wiley and Sons
Year: 1996
Tongue: French
Weight: 717 KB
Volume: 65
Category: Article
ISSN: 0020-7136
DOI: 10.1002/(sici)1097-0215(19960315)65:6<858::aid-ijc25>3.0.co;2-z

No coin nor oath required. For personal study only.

✦ Synopsis

The expression of the proto-oncogene, translation factor elF-4E, was examined in breast-cell lines: 5 carcinomas and 2 normal. At the protein level, elF-4E was 10 times higher in the carcinoma lines than in normal cells, which is comparable to the level found in breast-cancer biopsies. The elevation appears to be due to increased transcription, since the elF-4E mRNA was correspondingly increased. These results demonstrate that cells isolated from naturally occurring breast carcinomas maintain an elevated expression of the factor. Turnover rates for elF-4E (mRNA and protein) were determined for normal and cancer cells. We found that elF-4E mRNA is relatively stable during an 8-hr incubation with Actinomycin D, but the half-life of the protein is fairly short ( ~4 . 5 hr). This suggests that, in proliferating cells, elF-4E may be turning over rapidly, possibly to fine-tune the changes in translation rates which occur during the cell cycle.

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