𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Overexpression and Purification of Elongation Factor 3 fromSaccharomyces cerevisiae

✍ Scribed by Ravi Kambampati; Kalpana Chakraburtty

Book ID
115645164
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
146 KB
Volume
10
Category
Article
ISSN
1046-5928

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Purification and properties of polyphosp
Purification and properties of polyphosphatase fromSaccharomyces cerevisiae cytosol
✍ Andreeva, Nadezhda; Kulakovskaya, Tatjana; Sidorov, Igor; Karpov, Alexander; Kul πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 144 KB

A homogenous polyphosphatase preparation was obtained from Saccharomyces cerevisiae cytosol with a 3β€’8% yield and 3540-fold purification. The enzyme hydrolysed polyphosphate (polyP) with various chain lengths, including polyP 3 , and split P i off the end of the chain. It was inactive with respect t

Purification and characterization of a (
Purification and characterization of a (R)-2,3-butanediol dehydrogenase fromSaccharomyces cerevisiae
✍ JΓΌrgen Heidlas; Roland Tressl πŸ“‚ Article πŸ“… 1990 πŸ› Springer 🌐 English βš– 684 KB

A NAD-dependent (R)-2,3-butanediol dehydrogenase (EC 1.1.1.4), selectively catalyzing the oxidation at the (R)-center of 2,3-butanediol irrespective of the absolute configuration of the other carbinol center, was isolated from cell extracts of the yeast Saccharomyces cerevisiae. Purification was ach

Partial purification and some properties
Partial purification and some properties of lipoxygenase fromSaccharomyces cerevisiae
✍ B. Bisakowski; S. Kermasha; C. Schuepp πŸ“‚ Article πŸ“… 1995 πŸ› Springer 🌐 English βš– 236 KB

A partially purified lipoxygenase extract was obtained from the yeast Saccharomyces cerevisiae by precipitation with solid (NH4)SO4 at 20% to 80% saturation. The enzyme had two pH optima, at pH 8.0 and 10.0, with respective apparent K m values of 13 and 9.5 ΞΌM. At both pH optima, the lipoxygenase de

Partial purification and characterizatio
Partial purification and characterization of succinyl-CoA synthetase fromSaccharomyces cerevisiae
✍ H. Schwartz; H. -O. Steitz; F. Radler πŸ“‚ Article πŸ“… 1983 πŸ› Springer Netherlands 🌐 English βš– 511 KB

Succinyl-CoA synthetase from Saccharomyces cerevisiae was partially purified (20-fold) with a yield of 44%. The Michaelis-Menten constants were determined: Km (succinate) = 17 mM; Km (ATP) = 0.13 mM; Km (CoA) = 0.03 mM. The succinyl-CoA synthetase has a molecular weight of about 80000 dalton (as det