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Osmoprotective Effect of Glycine Betaine on Thrombopoietin Production in Hyperosmotic Chinese Hamster Ovary Cell Culture: Clonal Variations

โœ Scribed by Tae Kyung Kim; Joon Soo Ryu; Joo Young Chung; Min Soo Kim; Gyun Min Lee

Book ID
109386170
Publisher
American Institute of Chemical Engineers
Year
2000
Tongue
English
Weight
93 KB
Volume
16
Category
Article
ISSN
8756-7938

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Osmoprotective effect of glycine betaine
Osmoprotective effect of glycine betaine on foreign protein production in hyperosmotic recombinant Chinese hamster ovary cell cultures differs among cell lines
โœ Joon Soo Ryu; Tae Kyung Kim; Joo Young Chung; Gyun Min Lee ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 166 KB ๐Ÿ‘ 2 views

When three recombinant Chinese hamster ovary (rCHO) cell lines, CHO/dhfr-B22-4, CS13-1.00\*, and CS13-0.02\*, were cultivated in hyperosmolar media resulting from NaCl addition, their specific foreign protein productivity increased with medium osmolality. However, due to a simultaneous suppression o