Asparagine linked (N-linked) glycosylation is an important modification of recombinant proteins, because the attached oligosaccharide chains can significantly alter protein properties. Potential glycosylation sites are not always occupied with oligosaccharide, and site occupancy can change with the
Effects of phosphatidic acid on recombinant protein production by Chinese hamster ovary cells in serum-free culture
โ Scribed by Kentaro Sakai; Toshio Matsunaga; Chisa Hayashi; Hideki Yamaji; Hideki Fukuda
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 173 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1369-703X
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The development of serum-and protein-free Chinese hamster ovary (CHO) cell cultures is a high priority for the production of biopharmaceuticals. Proteinfree competent CHO cells lines have been previously constructed by two different methods-metabolic engineering with cell-cycle regulatory proteins a
When three recombinant Chinese hamster ovary (rCHO) cell lines, CHO/dhfr-B22-4, CS13-1.00\*, and CS13-0.02\*, were cultivated in hyperosmolar media resulting from NaCl addition, their specific foreign protein productivity increased with medium osmolality. However, due to a simultaneous suppression o
Although serum-free media have been widely used in mammalian cell culture for therapeutic protein production, the effects of serum-substitutes on product quality have not been extensively examined. This study observed an adverse effect of Primatone RL, an animal tissue hydrolysate commonly used as a