Organometallic complexes with biological molecules. XIV. Biological activity of dialkyl and trialkyltin(IV) [meso-tetra(4-carboxy- phenyl)porphinate] derivatives

✍ Scribed by C. Mansueto; E. Puccia; F. Maggio; R. Di Stefano; T. Fiore; C. Pellerito; F. Triolo; Professor L. Pellerito

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 221 KB
• Volume: 14
• Category: Article
• ISSN: 0268-2605
• DOI: 10.1002/(sici)1099-0739(200005)14:5<229::aid-aoc977>3.0.co;2-8

✦ Synopsis

The effects of several organotin(IV) mesotetra(4-carboxyphenyl)porphinate] derivatives with the general formula (R 2 Sn) 2 TPPC and (R 3 Sn) 4 TPPC (R = Me, Bu, Ph) were tested in vivo on ascidian embryonic development. Embryos at the two-cell stage were incubated in 1 Â 10 À5 or 1 Â 10 À7 M solutions of various compounds. The ligand, [meso-tetra(4-carboxyphenyl)porphine] (H 4 TPPC) was toxic at 1 Â 10 À5 M, because development was blocked at an early gastrula stage, whereas 1 Â 10 À7 M H 4 TPPC allowed the eggs to develop up to the larva stage. The most toxic among the tested compounds was tributyltin(IV) [meso-tetra (4-carboxyphenyl)porphinate], (Bu 3 Sn) 4 TPPC, since the fertilized eggs were unable to divide into two cells, even at a concentration of 1 Â 10 À7 M. To correlate this embryonic arrest with the metabolic pathway, and especially to understand why cellular organelles first underwent chemical damage, 10 À5 and 10 À7 M (Bu 3 Sn) 4 TPPC-cultured fertilized eggs were tested for DNA, RNA, protein, glucose, lipid and ATP contents, comparing the values obtained with those of control culture fertilized egg contents. The higher concentration (1 Â 10 À5 M) reduced the content of all the tested compounds, but the lower one (1 Â 10 À7 M), even if still unable to allow cleavage, reduced only the lipids and the ATP contents. A hypothesis concerning initial damage to mitochondrial membrane is proposed.