Organometallic complexes with biological molecules: XIII. Organotin(IV)[meso-tetra (4-carboxyphenyl)porphinate]s and the cell cycle: a flow-cytometric approach

✍ Scribed by F. Triolo; C. Pellerito; G. C. Stocco; T. Fiore; F. Maggio; L. Pellerito; R. Triolo

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 138 KB
• Volume: 13
• Category: Article
• ISSN: 0268-2605
• DOI: 10.1002/(sici)1099-0739(199910)13:10<733::aid-aoc923>3.0.co;2-m

✦ Synopsis

The cytotoxic derivatives diorganotin(IV) and triorganotin(IV) [meso-tetra(4-carboxyphenyl)porphinates, with stoichiometries [R 2 Sn] 2 TPPC and [R 3 Sn] 4 TPPC [R = Me, Bu, Ph; TPPC 4À = meso-tetra(4-carboxyphenyl)porphinate 4À ], namely bis[dimethyltin(IV)], bis[dibutyltin(IV)], bis[diphenyltin(IV)], tetra[trimethyltin(IV)], tetra[tributyltin(IV)] and tetra[triphenyltin(IV)] [meso-tetra(4-carboxyphenyl)porphinate]s, have been used to investigate their effects on the cultured human kidney cell cycle in order to understand further the origin of cell-growth inhibition induced by the above-mentioned chemicals. The cell-cycle-dependent DNA content distribution of cultured cells exposed to these compounds has been analyzed through flow cytometry, a potent technique capable of probing several aspects of drug-induced cytotoxicity. Cultured human kidney cells have been used as a model system, on the premise of greater physiological similarity to the human situation in vivo.