𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Organic ion imaging of biological tissue with secondary ion mass spectrometry and matrix-assisted laser desorption/ionization

✍ Scribed by Peter J. Todd; T. Gregory Schaaff; Pierre Chaurand; Richard M. Caprioli

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
787 KB
Volume
36
Category
Article
ISSN
1076-5174

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Organic secondary ion mass spectrometry (SIMS) and matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry can be used to produce molecular images of samples. This is achieved through ionization from a clearly identified point on a flat sample, and performing a raster of the sample by moving the point of ionization over the sample surface. The unique analytical capabilities of mass spectrometry for mapping a variety of biological samples at the tissue level are discussed. SIMS provides information on the spatial distribution of the elements and low molecular mass compounds as well as molecular structures on these compounds, while MALDI yields spatial information about higher molecular mass compounds, including their distributions in tissues at very low levels, as well as information on the molecular structures of these compounds. Application of these methods to analytical problems requires appropriate instrumentation, sample preparation methodology, and a data presentation usually in a three‐coordinate plot where x and y are physical dimensions of the sample and z is the signal amplitude. The use of imaging mass spectrometry is illustrated with several biological systems. Copyright © 2001 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Positive- and negative-ion matrix-assist
Positive- and negative-ion matrix-assisted laser desorption/ionization mass spectrometry of saccharides
✍ Chunyan Hao; Xiuli Ma; Shiping Fang; Zhiqiang Liu; Shuying Liu; Fengrui Song; Ju 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 122 KB 👁 2 views

## Matrix -assisted laser desorption/ionization (MALDI) mass spectrometry (MS) of oligosaccharides and polysaccharides has been investigated in detail. It is demonstrated that cationized species of oligosaccharides, [MNa] and [MK] , are dominant products under the MALDI condition, and negative ion

Ion Formation and Fragmentation of Sinap
Ion Formation and Fragmentation of Sinapinic Acid in Electron Ionization, Liquid Secondary Ion and Matrix-assisted Laser Desorption/Ionization Mass Spectrometry
✍ T. Kosaka; T. Kinoshita; M. Takayama 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 329 KB 👁 2 views

The ion formation and fragmentation of sinapinic acid, which is a typical matrix material for matrix-assisted laser desorption/ionization (MALDI), have been investigated by using electron ionization @I), liquid secondary ion (LSI) and MALDI methods. Collisionally activated dissociation (CAD) spectra

Inline pneumatically assisted atmospheri
Inline pneumatically assisted atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry
✍ Arti T. Navare; Facundo M. Fernández 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 347 KB

## Abstract Atmospheric pressure (AP) matrix‐assisted laser desorption/ionization (MALDI) is known to suffer from poor ion transfer efficiencies as compared to conventional vacuum MALDI (vMALDI). To mitigate these issues, a new AP‐MALDI ion source utilizing a coaxial gas flow was developed. Nitroge

Ionization mechanisms in matrix-assisted
Ionization mechanisms in matrix-assisted laser desorption/ionization mass spectrometry: contribution of pre-formed ions
✍ Edda Lehmann; Richard Knochenmuss; Renato Zenobi 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 316 KB 👁 2 views

The mechanisms of ion formation in matrix-assisted laser desorption/ionization mass spectrometry are still largely unknown. Ions may exist as pre-formed species in the solid state, or may form by ion-molecule reactions initiated by the laser shot, or, most probably, they may originate from a combina

Matrix-assisted laser desorption/ionizat
Matrix-assisted laser desorption/ionization mass spectrometry of noncovalent protein-transition metal ion complexes
✍ Salih, Bekir; Masselon, Christophe; Zenobi, Renato 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 319 KB 👁 2 views

Transition metal ion complexes with proteins and peptides are important in many areas of analytical and biological chemistry. We used positive and negative ion MALDI-MS to detect complexes with Cu and Ni ions, and show that the speciÐc and non-speciÐc transition metal ion-peptide complexes can be di

Biological tissue imaging with time-of-f
Biological tissue imaging with time-of-flight secondary ion mass spectrometry and cluster ion sources
✍ Alain Brunelle; David Touboul; Olivier Laprévote 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 873 KB

## Abstract Time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS) using liquid metal ion guns (LMIGs) is now sensitive enough to produce molecular‐ion images directly from biological tissue samples. Primary cluster ions strike a spot on the sample to produce a mass spectrum. An image of this sa