An inexpensive minispinner vessel for the suspension culture of mammalian cell lines in 2 to 20 ml of culture medium is described. It is made from a commercial glass or low-pressure polyethylene scintillation vial equipped with an easy-to-build stir bar assembly suspended from the cap.
Orbital shaker technology for the cultivation of mammalian cells in suspension
β Scribed by Natalie Muller; Philippe Girard; David L. Hacker; Martin Jordan; Florian M. Wurm
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 283 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract For the cultivation of mammalian cells on microcarriers a minimum inoculum concentration is required to initiate cell attachment and subsequent cell growth. A critical cell number model has been proposed to elucidate the mechanism of the inoculum requirement. In this model it was hypoth
Inoculation and growth conditions for the large-scale expansion of mammalian neural stem cells (NSC) have been determined. We examined suspension culture bioreactors of murine NSC, and concluded that the oxygen level should be kept high (20%), and the osmolarity of the medium should be kept low (bel