Optimum conditions for electrospray mass spectrometry of a monoclonal antibody
β Scribed by Keiryn L. Bennett; Larry A. Hick; Roger J. W. Truscott; Margaret M. Sheil; Suzanne V. Smith
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 297 KB
- Volume
- 30
- Category
- Article
- ISSN
- 1076-5174
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The location of the disulfide bonds in a recombinant monoclonal antibody was confirmed by matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) and electrospray ionization (ESI) mass spectrometry (MS). A non-reduced Endoproteinase Lys-C (Endo Lys-C) digest of the antibody was analyz
We describe a probabilistic peptide fragmentation model for use in protein databank searching and __de novo__ sequencing of electrospray tandem mass spectrometry data. A probabilistic framework for tuning of the model using a range of well-characterized samples are introduced. We present preliminary
Electrospray ionization (ESI) is nowadays the most important technique for on-line liquid chromatography/mass spectrometry (LC/MS) coupling. Di β erent ESI probe designs including the microESI and pneumatically assisted ESI (ionspray) interfaces allow the introduction of sample at Γow-rates ranging f