Comparison of conventional, narrow-bore and capillary liquid chromatography/mass spectrometry for electrospray ionization mass spectrometry: practical considerations

✍ Scribed by Abian, J.; Oosterkamp, A. J.; Gelpí, E.

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 315 KB
• Volume: 34
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/(sici)1096-9888(199904)34:4<244::aid-jms775>3.0.co;2-0

✦ Synopsis

Electrospray ionization (ESI) is nowadays the most important technique for on-line liquid chromatography/mass spectrometry (LC/MS) coupling. Di †erent ESI probe designs including the microESI and pneumatically assisted ESI (ionspray) interfaces allow the introduction of sample at Ñow-rates ranging from a few hundred nl min-1 to 1-2 ml min-1. In this paper, an overview of the di †erent LC/ESIMS devices is presented from the point of view of their Ñow compatibility. Several parameters for LC/ESIMS miniaturization and its e †ects on sensitivity are considered from the practical point of view. Low-Ñow ESI requires the use of narrow-bore and capillary columns. Sample preconcentration microdevices are recommended in order to circumvent some miniaturization drawbacks such as the low optimum injection volume and the low column capacity. Some considerations on fused-silica microcolumn and microESI needle construction are also presented.