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Determination of 8-Hydroxydeoxyguanosine in Biological Tissue by Liquid Chromatography/Electrospray Ionization-Mass Spectrometry/Mass Spectrometry

✍ Scribed by Jose Serrano; Carlos M. Palmeira; Kendall B. Wallace; Douglas W. Kuehl

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 340 KB
Volume: 10
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199611)10:14<1789::aid-rcm752>3.0.co;2-6

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✦ Synopsis

A method based on liquid chromatography (LC) combined with electrospray ionization tandem mass spectrometry for the analysis of the frequency of formation of 8-hydroxydeoxyguanosine (8OHdGuo), a biomarker of oxidative damage to DNA, has been developed. This analytical technique has the advantage over gas chromatography combined with mass spectrometry of not requiring analyte derivatization, and over LC combined with ultraviolet and electrochemical detection of being chemospecific The method was evaluated by determining increased frequency of formation of SOHdGuo in male Sprague-Dawky rats given a single intraperitoneal dose of Adriamycin" compared to control rats. Detection of one oxidized deoxyguanosine per 5 x 10s deoxyguanosines was readily achieved.

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