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Optimizing the generation of random amplified polymorphic DNAs in chrysanthemum

✍ Scribed by K. Wolff; E. D. Schoen; J. Peters-Van Rijn

Publisher
Springer
Year
1993
Tongue
English
Weight
398 KB
Volume
86
Category
Article
ISSN
0040-5752

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✦ Synopsis

Many conditions of the RAPD reaction procedure may influence the result. This paper presents rapid detection of influential factors with a fractional factorial experiment. A more extensive study of these factors is also presented. Polymerase brand, thermal cycler brand, annealing temperature, and primer, are important factors in obtaining good DNA yields and optimal fragment patterns. Each primer has its optimal annealing temperature, and this is not correlated with the GC content of the primer. Optimal species-primer combinations have to be found by trial and error.

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