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Optimization of random amplified polymorphic DNA techniques for its use in genetic studies of Trichomonas vaginalis isolates

✍ Scribed by Jorge Fraga; Lázara Rojas; Idalia Sariego; Carlos A Sarrı́a

Publisher: Elsevier Science
Year: 2002
Tongue: English
Weight: 146 KB
Volume: 2
Category: Article
ISSN: 1567-1348
DOI: 10.1016/s1567-1348(02)00086-2

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✦ Synopsis

The random amplified polymorphic DNA (RAPD) technique is a simple method to detect DNA polymorphism. Several factors can affect the amplification profiles causing the presence of false bands and assay non-reproducibility. In this study, we analyzed the effect of changing concentrations of the primer, template DNA and Taq DNA polymerase with the goal of determining their optimum concentration for the standardization of the RAPD technique for genetic studies of Trichomonas vaginalis, a parasite that is of major epidemiological relevance in Cuba.

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