Optimized determination of indole derivatives by high-performance liquid chromatography with selective detection

✍ Scribed by Roman Kysilka; Milan Wurst

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 476 KB
• Volume: 446
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94447-9

✦ Synopsis

A procedure for the optimum separation and determination of indole derivatives was developed, based on a generally applicable algorithm for computer processing of experimental data. Experiments were performed on a Separon SGX Cl8 column with a mobile phase of citrate phosphate buffer (pH 3. l), containing various amounts of ethanol. A tandem combination of an UV-photometric detector at 267 nm and an electrochemical detector with a wall-jett cell was used. The detection limit was lO-'O g for certain indole derivatives. The optimization by means of the computer program consists of the following steps: display of the dependence of the capacity factors on the ethanol content in the mobile phase, display of the course of the optimization function and construction of separation maps and simulation of chromatograms.