Optimization of selectivity, detectability and analysis time in high-performance liquid chromatography

✍ Scribed by S. Ahuja

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 590 KB
• Volume: 499
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96995-4

✦ Synopsis

The resolution requirements in pharmaceutical analysis by high-performance liquid chromatograpy are frequently higher than those for other multicomponent separations where the components are present in similar proportion. Furthermore, it is often necessary to separate a large number of components most of which are present at very low levels and have unknown structures. This places extraordinary requirements in terms of selectivity of separation and resolution of minor components as they may not be detected because of variability of stationary phase, improper selectivity evaluations,

or poor resolution at the tail end of the major peak. Discussion with respect to resolution, detectability/quantitation, and analysis time is included.