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Selectivity tuning of serially coupled columns in high performance liquid chromatography

✍ Scribed by Welsch, Thomas ;Dornberger, Uwe ;Lerche, Dietmar

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
621 KB
Volume
16
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

The tuning of selectivity by changing the flow rate has been investigated in HPLC: two columns with different retention characteristics were coupled in series via a T‐piece and the relative retention of components chromatographed on the system were changed by varying the individual flow rates in the coupled columns. The flow rate alteration was performed by adding a second flow after the first column. The flow rate ratio necessary for optimum resolution can be easily calculated on the basis of the capacity factors measured on the individual columns. The performance of this method for adjusting selectivity has been demonstrated by using different column combinations to separate several mixtures containing chlorophenols, nitroaromatic compounds, and aromatic hydrocarbons.

πŸ“œ SIMILAR VOLUMES

High-performance liquid chromatography o
High-performance liquid chromatography of proteins on short capillary columns
✍ Mal'tsev, V. G. ;Nasledov, D. G. ;Trushin, S. A. ;Tennikova, T. B. ;Vinogradova, πŸ“‚ Article πŸ“… 1990 πŸ› John Wiley and Sons 🌐 English βš– 400 KB

## Abstract A microchromatographic procedure for highly sensitive analysis of proteins has been developed by using short capillary columns with reverse‐phase, ion‐exchange, and hydrophobic interaction sorbents. The high analytical mass sensitivity on the level of several nanograms was attained by t