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High-performance liquid chromatography of proteins on short capillary columns

✍ Scribed by Mal'tsev, V. G. ;Nasledov, D. G. ;Trushin, S. A. ;Tennikova, T. B. ;Vinogradova, L. V. ;Volokitina, I. N. ;Zgonnik, V. N. ;Belenkii, Boris G.

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
400 KB
Volume
13
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

A microchromatographic procedure for highly sensitive analysis of proteins has been developed by using short capillary columns with reverse‐phase, ion‐exchange, and hydrophobic interaction sorbents. The high analytical mass sensitivity on the level of several nanograms was attained by the miniaturization of the column and the optimization of gradient steepness.

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