Particle-loaded (3 lm, octadecylsilica) monolithic sol-gel columns have been prepared and selected characteristics measured. Several electrical properties may be calculated from simple current measurements in the column as a whole. Resistivity in the packed segment is approximately three times that
Advances in capillary electrochromatography and micro-high performance liquid chromatography monolithic columns for separation science
β Scribed by Cristina Legido-Quigley; Nicola D. Marlin; Virginie Melin; Andreas Manz; Norman W. Smith
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 290 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0173-0835
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β¦ Synopsis
Over the last decade, monoliths or continuous beds have emerged as an alternative to traditional packed-bed columns for use in capillary electrochromatography (CEC) and micro-high performance liquid chromatography (micro-HPLC). Monolithic columns can be divided into two categories: silica-based monolithic columns and rigid organic polymer-based monolithic columns resulting from the polymerization of acrylamide, styrene, acrylate or methacrylate monomers. In this paper, the chemistry and most recent applications of these various types of monoliths in both CEC and micro-HPLC are presented.
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## Abstract In this report, microcolumn separation schemes involving monolithic capillary columns with immobilized lectins, and relevant to nanoglycomics/nanoproteomics were introduced. Positive and neutral monoliths based on poly(glycidyl methacrylateβ__co__βethylene dimethacrylate) were designed